YSI 2900/2950 Integration

The Flownamics Seg-Flow® PS Fully Automated Sampling System and Director™ Liquid Handler, integrated with the YSI 2900/2950 Series Biochemistry Analyzers, provide a fully automated solution for bioprocess sampling, nutrient and metabolite analysis, data management, and process control.

For more information on the YSI 2900/2950 Series Biochemistry Analyzers, please visit the YSI Life Sciences website.

YSI and Seg-Flow PS Integration

YSI, Director and Seg-Flow PS Integration

Flownamics 82-IM

Description

System Configuration and Capacity

The integrated system automatically delivers samples from bioreactors or process streams to the YSI analyzer, acquires analytical results, and associates those results with the appropriate sampling event. This provides more frequent process data while reducing the manual effort required for routine sampling and analysis.

YSI 2900 Series analyzers are widely used for cell culture and fermentation applications and can measure nutrients and metabolites including glucose, lactate, glutamine, glutamate, ammonium, potassium, glycerol, ethanol, and other chemistries depending on analyzer configuration. The YSI 2900D supports up to two chemistries, while the YSI 2950 platform can be configured for up to six.

The Seg-Flow® PS provides automated sample withdrawal and transfer from bioreactors or process streams to the YSI 2900/2950 Series Biochemistry Analyzer. For applications requiring additional vessels or analytical destinations, multiple Seg-Flow PS modules can be combined with the Director™ Liquid Handler to create a scalable automated sampling platform.

The Director coordinates sample routing between the Seg-Flow PS modules and connected analytical instruments. A system can be configured to support up to 16 bioreactors or process streams and route samples to as many as four analyzers or fraction-collection destinations, allowing multiple analytical measurements to be incorporated into a single automated sampling workflow.

The Seg-Flow PS uses a disposable fluid path and can transfer samples to analytical equipment located away from the bioreactor. Current Seg-Flow PS delivery-line options include 10-, 25-, and 50-foot lengths, providing flexibility when analyzers are centralized or located outside the immediate bioreactor area.

Automated Sampling and Sample Management

The Seg-Flow PS automatically withdraws a user-defined sample from each configured bioreactor or process stream and transfers it to the YSI analyzer according to a programmed sampling schedule. Individual sampling frequencies can be established based on process requirements, allowing critical vessels or process stages to be monitored more frequently.

Cell-free sampling can be performed using Flownamics FISP® Sampling Probe technology. YSI currently lists the FISP as an option for its online bioprocess monitoring applications, with a 0.2-micron membrane providing a sterile barrier between the culture and sampling system.

The current Seg-Flow PS platform can also be configured for cell-containing or cell-free sample withdrawal depending on the application and approved sampling interface. Its disposable fluid path helps reduce manual handling and cross-contamination risk between sampling operations.

When combined with the Director, samples can also be directed to additional analytical instruments or retained for offline testing, creating a single automated sampling platform capable of supporting multiple analytical workflows.

YSI Analyzer Integration and Automated Functions

The Flownamics YSI interface was developed to coordinate sampling with the YSI 2900/2950 analyzer and automate functions that would otherwise require operator interaction.

The integration supports automated YSI status checks, including monitoring of buffer, waste, calibration standards, probe calibration status, modules, and analyzer system status. Probe-specific calibration and module-specific measurements can also be initiated through the integrated workflow.

YSI configuration within the Flownamics interface allows individual probes to be enabled and configured for the appropriate chemistry, measurement units, buffer location, and calibration-standard location. Fluid detection can also be enabled when required.

The YSI analyzer’s modular configuration allows the analytical system to be tailored to the process. Depending on the installed probes and modules, supported chemistries can include glucose, lactate, glutamine, glutamate, ammonium, potassium, ethanol, methanol, sucrose, galactose, lactose, choline, glycerol, hydrogen peroxide, and other supported measurements.

YSI’s analyzer fluidics are designed for bioprocess samples and to resist clogging even at relatively high cell densities, allowing the analyzer to support a wide variety of cell culture and fermentation applications.

Data Integration and Process Control

Analytical results generated by the YSI 2900/2950 are automatically acquired by the Flownamics system and associated with the appropriate bioreactor or process stream. This eliminates manual transcription and creates a consistent connection between the sample event, vessel, and analytical result.

Results can be displayed and trended for real-time process monitoring and can also be used as inputs for automated feed-control strategies. For example, measured glucose or other nutrient concentrations can be used to support feedback-based process control. Flownamics’ established YSI integration has historically supported both automated data acquisition and feed-control functions.

Through Flownamics OPC connectivity, analytical and sampling data can be transferred to compatible supervisory control and data acquisition systems, historians, distributed control systems, or bioprocess management platforms. This provides centralized access to analytical data and supports integration of at-line measurements into broader process-control strategies.

YSI also identifies the 2900 Series as a process analytical technology for bioprocess monitoring, with connectivity and data-management options that allow analytical results to be communicated to process-control and data-history systems.

Benefits of Automated Integration

Integrating the Seg-Flow PS and Director with the YSI 2900/2950 automates sample collection, transfer, analysis, data acquisition, and optional process-control functions.

By removing many of the repetitive manual steps associated with routine nutrient and metabolite measurements, the system can provide more frequent analytical data while reducing operator involvement. Automated sampling also helps maintain consistent sample timing and handling from vessel to vessel.

The result is a scalable process analytical solution that can be used to improve process visibility, accelerate response to changing culture conditions, and provide the data needed for more advanced bioprocess monitoring and control.

Product Specifications

Analyzer Compatibility	YSI 2900/2950 Series Biochemistry Analyzers
YSI 2900 Configuration	Up to two chemistries, depending on installed probes and analyzer configuration
YSI 2950 Configuration	Up to six chemistries, depending on installed modules and probes
Automated YSI Functions	Analyzer status checks, probe-specific calibration, module-specific measurement, and chemistry configuration
Analyzer Status Monitoring	Buffer, waste, calibration standards, probe calibration, modules, and analyzer status
Chemistry Configuration	Independent probe activation and configuration of chemistry, units, buffer location, and calibration-standard location
Sampling	Automated sampling from bioreactors or process streams using the Seg-Flow PS
Sample Volume	User-defined; current Seg-Flow PS minimum sample-volume configuration is 0.25 mL
Sample Transfer Distance	Seg-Flow PS delivery lines available in 10-, 25-, and 50-foot lengths
Sampling Modes	Cell-free sampling with FISP technology; other sampling configurations available based on application
Multi-Vessel Configuration	Scalable configurations supporting up to 16 bioreactors/process streams
Analytical Destinations	Up to four analyzers or fraction-collection destinations with Director-based configurations
Data Acquisition	Automated acquisition and vessel/sample-event association of YSI analytical results
Process Control	YSI analytical results can be used to support automated feed-control strategies
Data Integration	OPC connectivity to compatible process-control systems, historians, and bioprocess management platforms
Fluid Path	Disposable Seg-Flow sampling fluid path

Features & Benefits

Fully automated bioprocess sampling and analysis — Integrates the Seg-Flow® PS Fully Automated Sampling System and Director™ Liquid Handler with the YSI 2900/2950 Series Biochemistry Analyzers.
Automated nutrient and metabolite monitoring — Provides automated delivery of bioprocess samples for measurement of glucose, lactate, glutamine, glutamate, and other supported chemistries.
Flexible analyzer configurations — The YSI 2900 platform supports up to two chemistries, while the YSI 2950 can be configured for up to six chemistries.
Automated YSI status monitoring — Checks analyzer conditions including buffers, waste, calibration standards, probe calibration, modules, and system status.
Automated calibration and measurement — Supports probe-specific calibration and module-specific measurement functions without routine operator intervention.
Simple chemistry configuration — Allows individual probes to be activated and configured for chemistry, measurement units, buffer locations, and calibration-standard locations.
Scalable multi-bioreactor sampling — Seg-Flow PS and Director configurations can support automated workflows involving up to 16 bioreactors or process streams.
Supports multiple analytical devices — Director-based systems can route automated samples to as many as four analyzers or fraction-collection destinations through a single sampling platform.
Flexible analyzer placement — Sample-transfer lines are available in lengths up to 50 feet, allowing analytical equipment to be centralized or located away from the bioreactors.
Cell-free sampling capability — FISP® Sampling Probes provide automated microfiltered sampling while maintaining a sterile barrier to the bioreactor.
Disposable sampling fluid path — Reduces fluid-path maintenance and helps minimize cross-contamination risk between sampling operations.
Customizable sampling schedules — Sampling volume and frequency can be configured based on individual process requirements.
Automated data acquisition — Analytical results are automatically associated with the appropriate bioreactor and sampling event.
Real-time process monitoring — More frequent measurements provide increased visibility into nutrient consumption and metabolite accumulation during cell culture and fermentation.
Automated feed-control capabilities — Analytical measurements such as glucose can be incorporated into feedback-based feeding strategies.
OPC connectivity — Analytical and process data can be transferred to compatible supervisory control and data acquisition systems, historians, and bioprocess management platforms.
Reduced manual sampling — Minimizes repetitive operator activities associated with sample withdrawal, transfer, analyzer loading, and data entry.
Supports unattended operation — Automated sampling schedules can continue during evenings, nights, and weekends, providing more consistent process data while reducing off-shift sampling requirements.

Literature

YSI 2900/2950 Integration

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YSI 2900/2950 Integration