FISP® Cell-Free Sampling Probe
Flownamics® developed the FISP® sampling probe in 1996. The FISP® sampling probe is capable of withdrawing sterile, cell-free samples from fermenters and bioreactors. FISP® allows direct on-line sample transfer to a variety of sampling systems (Seg-Flow), analyzers (such as biochemistry and HPLC systems) and fraction collectors for off-line analysis. FISP® is constructed of 316 stainless steel and fitted with a tubular, micro-porous membrane. The probes come in a variety of sizes (6, 12, 19 and 25 mm ports) to accommodate laboratory, pilot or industrial scale vessels. FISP® has been and continues to be widely utilized in the biotechnology and bioprocess industries for fermentation and cell-culture since its introduction to the market.
- Description
- Product Specifications
- D-Series FISP Probe
- F-Series FISP Probe
- Features of the FISP®
- Operation of the FISP®
- Literature
Description
FISP probes can be used for submerged feeding/sterile media addition
- Fast & Easy Setup: Removes the autoclaving reagents step in the process
- Eliminates risk of exposure to hazardous, volatile substances
- 0.2u ceramic membrane provides sterile barrier
Product Specifications
| Specifications | See D-Series and F-Series Probe Tabs for each model's specifications |
|---|
D-Series FISP Probe
- Available For 19 & 25 mm Ports: Standard and Safety
- Fits Into the Fermenter / Bioreactor Side Port
- Dead Volume: Approximately 0.24 to 0.44 ml
- Used in PD to Production
- GMP Validated
- SIP & CIP Compatible
- Immersion Depths: 90 and 115 mm
- Standard membrane pore size: 0.2 μm
- Stock Membrane Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, & 75.0 μm
- Custom Pore Sizes Available
F-Series FISP Probe
- Available For 8, 12, and 19 mm Ports
- Fits Into the Fermenter / Bioreactor Top Port
- Dead Volume: Approximately 0.24 to 0.44 ml
- Can Be Used With SUBs
- Autoclavable
- Compatible With Kleenpak™ Connectors
- Immersion Depths: 120, 200, 310, and 410 mm
- Standard membrane pore size: 0.2 μm
- Stock Membrane Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, & 75.0 μm
- Custom Pore Sizes Available
Features of the FISP®
- Sterile (0.2 μm Filter), Cell-Free Sampling for On-Line or Off-Line Analysis
- Minimal Dead Volume for Consistent and Accurate Sampling, which Reduces Flush Time
- Use with Aerobic or Anaerobic Bacteria, Yeast, Fungi, Algae, Insect, and Mammalian Cell Cultures
- Can Be Used for Submerged Feeding/Sterile Media Addition
- Standard Membrane 0.2 μm
- Additional Stock Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, 75.0 μm (Custom Pore Sizes Available)
- Interfaces With Our Seg-Flow® Sampling System
Operation of the FISP®
The FISP® is inserted into a side or top port of the vessel. It is immersed into the fermentation broth or culture media and autoclaved or sterilized in-situ. A peristaltic pump (or the vessel’s own hydrostatic pressure) withdraws a sample through the tubular membrane into the probe’s stainless steel network of grooves. The sample flows through the network of grooves into a central channel where the cell-free sample can be directed to a sampling system (Seg-Flow), on-line analyzer, fraction collector, or sample container. Fluid flow rate is dependent on the viscosity of the media.
Flownamics® has been creating innovative, high quality solutions for the biotechnology and bioprocess industry for over 27 years.
Our products provide our users with the tools that they need to improve their processes through automation. This results in higher quality data, more time, and a better work/life balance by requiring less off shift and weekend workloads. Contact Flownamics® to see how our products can make a difference for your process.
Our products have been featured in a variety of industry publications. Please visit our publications page to learn more.
