FISP® Cell-Free Sampling Probe

Flownamics® developed the FISP® sampling probe in 1996.  The FISP® sampling probe is capable of withdrawing sterile, cell-free samples from fermenters and bioreactors.  FISP® allows direct on-line sample transfer to a variety of sampling systems (Seg-Flow), analyzers (such as biochemistry and HPLC systems) and fraction collectors for off-line analysis.  FISP® is constructed of 316 stainless steel and fitted with a tubular, micro-porous membrane.  The probes come in a variety of sizes (6, 12, 19 and 25 mm ports) to accommodate laboratory, pilot or industrial scale vessels.  FISP® has been and continues to be widely utilized in the biotechnology and bioprocess industries for fermentation and cell-culture since its introduction to the market. 

FISP® Cell-Free Sampling Probe

Flownamics® developed the FISP® sampling probe in 1996.  The FISP® sampling probe is capable of withdrawing sterile, cell-free samples from fermenters and bioreactors.  FISP® allows direct on-line sample transfer to a variety of sampling systems (Seg-Flow), analyzers (such as biochemistry and HPLC systems) and fraction collectors for off-line analysis.  

Image of the FISP F-Series cell-free sampling probe.

FISP® Cell-Free Sampling Probe

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FISP probes can be used for submerged feeding/sterile media addition

  • Fast & Easy Setup: Removes the autoclaving reagents step in the process
  • Eliminates risk of exposure to hazardous, volatile substances
  • 0.2u ceramic membrane provides sterile barrier

FISP flow animation
Header picture of the D-series FISP cell-free sampling probe.

D-Series FISP® Probe

  • Available For 19 & 25 mm Ports: Standard and Safety
  • Fits Into the Fermenter / Bioreactor Side Port
  • Dead Volume: Approximately 0.24 to 0.44 ml
  • Used in PD to Production
  • GMP Validated
  • SIP & CIP Compatible
  • Immersion Depths: 90 and 115 mm
  • Standard membrane pore size:  0.2 μm
  • Stock Membrane Pore Sizes:  0.45, 0.75, 1.0, 5.0, 25.0, & 75.0 μm
  • Custom Pore Sizes Available

Picture of the F-series FISP probe.

F-Series FISP® Probe

  • Available For 8, 12, and 19 mm Ports
  • Fits Into the Fermenter / Bioreactor Top Port
  • Dead Volume: Approximately 0.24 to 0.44 ml
  • Can Be Used With SUBs
  • Autoclavable
  • Compatible With Kleenpak™ Connectors
  • Immersion Depths: 120, 200, 310, and 410 mm
  • Standard membrane pore size:  0.2 μm
  • Stock Membrane Pore Sizes:  0.45, 0.75, 1.0, 5.0, 25.0, & 75.0 μm
  • Custom Pore Sizes Available

  • Sterile (0.2 μm Filter), Cell-Free Sampling for On-Line or Off-Line Analysis
  • Minimal Dead Volume for Consistent and Accurate Sampling, which Reduces Flush Time
  • Use with Aerobic or Anaerobic Bacteria, Yeast, Fungi, Algae, Insect, and Mammalian Cell Cultures
  • Can Be Used for Submerged Feeding/Sterile Media Addition
  • Standard Membrane 0.2 μm
  • Additional Stock Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, 75.0 μm (Custom Pore Sizes Available)
  • Interfaces With Our Seg-Flow® Sampling System

The FISP® is inserted into a side or top port of the vessel. It is immersed into the fermentation broth or culture media and autoclaved or sterilized in-situ. A peristaltic pump (or the vessel’s own hydrostatic pressure) withdraws a sample through the tubular membrane into the probe’s stainless steel network of grooves. The sample flows through the network of grooves into a central channel where the cell-free sample can be directed to a sampling system (Seg-Flow), on-line analyzer, fraction collector, or sample container. Fluid flow rate is dependent on the viscosity of the media. 

Contact us to see if the FISP® would be right for your application. 

Image of the FISP cell free sampling probe.