FISP® Cell-Free Sampling Probe
Flownamics® developed the FISP® sampling probe in 1996. The FISP® sampling probe is capable of withdrawing sterile, cell-free samples from fermenters and bioreactors. FISP® allows direct on-line sample transfer to a variety of sampling systems (Seg-Flow), analyzers (such as biochemistry and HPLC systems) and fraction collectors for off-line analysis. FISP® is constructed of 316 stainless steel and fitted with a tubular, micro-porous membrane. The probes come in a variety of sizes (6, 12, 19 and 25 mm ports) to accommodate laboratory, pilot or industrial scale vessels. FISP® has been and continues to be widely utilized in the biotechnology and bioprocess industries for fermentation and cell-culture since its introduction to the market.
FISP® Cell-Free Sampling Probe
Flownamics® developed the FISP® sampling probe in 1996. The FISP® sampling probe is capable of withdrawing sterile, cell-free samples from fermenters and bioreactors. FISP® allows direct on-line sample transfer to a variety of sampling systems (Seg-Flow), analyzers (such as biochemistry and HPLC systems) and fraction collectors for off-line analysis.

FISP probes can be used for submerged feeding/sterile media addition
- Fast & Easy Setup: Removes the autoclaving reagents step in the process
- Eliminates risk of exposure to hazardous, volatile substances
- 0.2u ceramic membrane provides sterile barrier


D-Series FISP® Probe
- Available For 19 & 25 mm Ports: Standard and Safety
- Fits Into the Fermenter / Bioreactor Side Port
- Dead Volume: Approximately 0.24 to 0.44 ml
- Used in PD to Production
- GMP Validated
- SIP & CIP Compatible
- Immersion Depths: 90 and 115 mm
- Standard membrane pore size: 0.2 μm
- Stock Membrane Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, & 75.0 μm
- Custom Pore Sizes Available

F-Series FISP® Probe
- Available For 8, 12, and 19 mm Ports
- Fits Into the Fermenter / Bioreactor Top Port
- Dead Volume: Approximately 0.24 to 0.44 ml
- Can Be Used With SUBs
- Autoclavable
- Compatible With Kleenpak™ Connectors
- Immersion Depths: 120, 200, 310, and 410 mm
- Standard membrane pore size: 0.2 μm
- Stock Membrane Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, & 75.0 μm
- Custom Pore Sizes Available
- Sterile (0.2 μm Filter), Cell-Free Sampling for On-Line or Off-Line Analysis
- Minimal Dead Volume for Consistent and Accurate Sampling, which Reduces Flush Time
- Use with Aerobic or Anaerobic Bacteria, Yeast, Fungi, Algae, Insect, and Mammalian Cell Cultures
- Can Be Used for Submerged Feeding/Sterile Media Addition
- Standard Membrane 0.2 μm
- Additional Stock Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, 75.0 μm (Custom Pore Sizes Available)
- Interfaces With Our Seg-Flow® Sampling System
The FISP® is inserted into a side or top port of the vessel. It is immersed into the fermentation broth or culture media and autoclaved or sterilized in-situ. A peristaltic pump (or the vessel’s own hydrostatic pressure) withdraws a sample through the tubular membrane into the probe’s stainless steel network of grooves. The sample flows through the network of grooves into a central channel where the cell-free sample can be directed to a sampling system (Seg-Flow), on-line analyzer, fraction collector, or sample container. Fluid flow rate is dependent on the viscosity of the media.
Contact us to see if the FISP® would be right for your application.
